Search results for "Accessory pigment"
showing 4 items of 4 documents
The use of spectral fluorescence methods to detect changes in the phytoplankton community
1998
In vivo fluorescence methods are efficient tools for studying the seasonal and spatial dynamics of phytoplankton. Traditionally the measurements are made using single excitation-emission wavelength combination. During a cruise in the Gulf of Riga (Baltic Sea) we supplemented this technique by measuring the spectral fluorescence signal (SFS) and fixed wavelength fluorescence intensities at the excitation maxima of main accessory pigments. These methods allowed the rapid collection of quantitative fluorescence data and chemotaxonomic diagnostics of the phytoplankton community. The chlorophyll a-specific fluorescence intensities (R) and the spectral fluorescence fingerprints were analysed toge…
Energy transfer and pigment composition in three chlorophyll b-containing light-harvesting complexes isolated from Mantoniella squamata (Prasinophyce…
1986
Light-harvesting Chl a/b protein complexes were isolated from the higher plant Sinapis alba, the green alga Chlorella fusca, and the prasinophycean alga Mantoniella squamata by mild gel electrophoresis. The energy transfer from chlorophyll b and the accessory xanthophyll was measured by means of fluoresence spectroscopy at 77 K. The pigment composition of the isolated antenna complexes was determined by high performance liquid chromatography in order to calculate the number of light absorbing molecules per chlorophyll a in the different light-harvesting complexes. These results were complemented by the quantitation of the pigments in total thylakoids as well as in the different electrophore…
Single amino acids in the lumenal loop domain influence the stability of the major light-harvesting chlorophyll a/b complex.
2004
The major light-harvesting complex of photosystem II (LHCIIb) is one of the most abundant integral membrane proteins. It greatly enhances the efficiency of photosynthesis in green plants by binding a large number of accessory pigments that absorb light energy and conduct it toward the photosynthetic reaction centers. Most of these pigments are associated with the three transmembrane and one amphiphilic alpha helices of the protein. Less is known about the significance of the loop domains connecting the alpha helices for pigment binding. Therefore, we randomly exchanged single amino acids in the lumenal loop domain of the bacterially expressed apoprotein Lhcb1 and then reconstituted the muta…
Changes in pigmentation of phytoplankton species during growth and stationary phase — consequences for reliability of pigment-based methods of biomas…
1991
In applied water ecology several methods for estimating the biomass or activity of phytoplankton depend on the proportion of accessory pigments (xanthophylls) to chlorophyll a. Therefore, changes in pigmentation during growth and stationary phase were investigated in four different species (Amphidinium klebsii, Euglena gracilis, Prymnesium parvum, Cryptomonas ovata) typical representatives of the major algal groups. The ratios of the different xanthophylls to chlorophyll a depended not only on the growth phase, but also on the species. InAmphidinium andEuglena, the ratio of xanthophylls to chlorophyll rises continuously during the growth phase and declined during the stationary phase. InPry…